br Herein we reported novel NPs
Herein, we reported novel NPs based on PEO-PBO-loaded LA-SN38 (EBNPs). The EBNPs with small size and narrow distribution, and could effectively control the release rate of SN38. Through in vitro cytotoxi-city and apoptosis assay, we demonstrated EBNPs were more effective in antitumor of human colon cancer cells. Furthermore, cell uptake assay indicated that EBNPs could reduce the U 0126 of macro-phages and promote the uptake of tumor cells. Pharmacokinetic and biodistribution studies confirmed EBNPs had the advantages of pro-longed blood circulation and enhanced tumor targeting ability. In vivo, EBNPs were more effective in inhibiting the growth of tumor than SNPs and CPT-1, and were well tolerated at the tested dose. In summary, EBNPs are shown as a promising candidate for delivery LA-SN38 for treatment of colorectal cancer. Moreover, EBNPs may be useful as a drug carrier for other drugs in other tumor types.
Declaration of Competing Interest
There are no conflicts to declare.
This work was supported by the National Natural Science Foundation of China [grant number 21602206].
L. Zhou, Deoxycholic acid-modified chitooligosaccharide/mPEG-PDLLA mixed mi-celles loaded with paclitaxel for enhanced antitumor efficacy, Int. J. Pharmaceut. 475 (2014) 60–68.  H. Wang, H. Xie, J. Wang, J. Wu, X. Ma, L. Li, X. Wei, L. Qi, P. Song, Z. Lin, Self-assembling prodrugs by precise programming of molecular structures that con-tribute distinct stability, pharmacokinetics, and antitumor efficacy, Adv. Funct. Mater. 25 (2015) 4956–4965.
 H.X. Wang, H.Y. Xie, J.P. Wu, X.Y. Wei, L. Zhou, X. Xu, S.S. Zheng, Structure-based rational design of prodrugs to enable their combination with polymeric nano-particle delivery platforms for enhanced antitumor efficacy, Angew. Chem. Int. Ed. 53 (2014) 11532–11537.  Y.Y. Yuan, L. Xu, S.Y. Dai, M. Wang, H.X. Wang, A facile supramolecular approach to fabricate multifunctional upconversion nanoparticles as a versatile platform for drug loading, in vivo delivery and tumor imaging, J. Mater. Chem. B 5 (2017)
 H.X. Wang, J.M. Chen, C. Xu, L.L. Shi, M. Tayier, J.H. Zhou, J. Zhang, J.P. Wu, Z.J. Ye, T. Fang, W.D. Han, Cancer nanomedicines stabilized by pi-pi stacking be-tween heterodimeric prodrugs enable exceptionally high drug loading capacity and
 H.X. Wang, J.P. Wu, K. Xie, T. Fang, C. Chen, H.Y. Xie, L. Zhou, S.S. Zheng, Precise engineering of prodrug cocktails into single polymeric nanoparticles for combina-tion cancer therapy: extended and sequentially controllable drug release, ACS Appl. Colloids and Surfaces B: Biointerfaces 181 (2019) 822–829
 K. Kataoka, A. Harada, Y. Nagasaki, Block copolymer micelles for drug delivery: design, characterization and biological significance, Adv. Drug Deliv. Rev. 64 (2012) 37–48.  H. Maeda, The enhanced permeability and retention (EPR) effect in tumor vascu-lature: the key role of tumor-selective macromolecular drug targeting, Adv. Enzyme Regul. 41 (2001) 189–207.  Q.Q. Ding, X.W. Xu, Y.Y. Yue, C.T. Mei, C.B. Huang, S.H. Jiang, Q.L. Wu, J.Q. Han, Nanocellulose-mediated electroconductive self-healing hydrogels with high strength, plasticity, viscoelasticity, stretchability, and biocompatibility toward multifunctional applications, ACS Appl. Mater. Interfaces 10 (2018) 27987–28002.
S.S. Zheng, H.X. Wang, Chemical derivatization of the anticancer agent cabazitaxel using a polyunsaturated fatty acid for safe drug delivery in vivo, J. Biomed. Nanotechnol. 14 (2018) 1853–1865.
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Biomedicine & Pharmacotherapy
journal homepage: www.elsevier.com/locate/biopha
Anticancer activity of synthetic ( ± )-kusunokinin and its derivative T
( ± )-bursehernin on human cancer cell lines
Thidarath Rattanabureea, Tienthong Thongpanchangb, Krittaphat Wongmac, Aman Tedasena, Yaowapa Sukpondmad, Potchanapond Graidista,e,
a Department of Biomedical Sciences, Faculty of Medicine, Prince of Songkla University, Songkhla, 90110, Thailand
b Department of Chemistry, Faculty of Science and Center of Excellence for Innovation in Chemistry, Mahidol University, Bangkok, 10400, Thailand
c General Sciences Program, Faculty of Education, Sakon Nakhon Rajabhat University, Sakon Nakhon, 47000, Thailand
d Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand
e The Excellent Research Laboratory of Cancer Molecular Biology, Prince of Songkla University, Songkhla, 90110, Thailand
Keywords: Kusunokinin is a potent lignan compound with a several biological properties including antitrypanosomal and
Kusunokin anticancer. In this study, ( ± )-kusunokinin and its derivative, ( ± )-bursehernin, were synthesized and in-